Pseudomyrmex triplarinus, a ant species found in the Peruvian rainforest, lives in the tree species Triplaris americana. The ants tunnel into the tree and use it as a "shelter and sustenance." In return for providing the ants these comforts, the ants will protect the tree from invaders, including other plants. These ants use chemical indicators to tell the difference between the host trees there living in and any competitor plants nearby. The ants use the surface wax on each tree to distinguish between the plants. Once a competitor plant is recognized, it is marked with a chemical to tell others to prune the competing plant. By pruning a plant that may compete for resources from the host plant, the ants guarantee the host plants survival. This symbiotic relationship with the trees was studies by scientists at Colorado State University. Two different experiments were done to "demonstrate how ants inhabiting the branches of T. americana trees recognize species-specific chemical signals embedded in the leaf surface." The scientists took a single T. americanan host tress and surrounded it by different species of trees. Over five weeks, researchers observed the ant behavior in pruning the trees. Also, they took leaves from several different species of trees (but also including the leafs of the T. americana), and attached them to the host tree with pins. Overall, the ants removed more leafs (pruned) that did not belong to the T. americana host tree. Researchers also wanted to know how specific the ants were in pruning non host trees. The took filter paper coated with the T. americana tree wax extract and its closely related species T. poeppigiana. Over observation, the ants attacked vigorously with those filters coated with the T. americana wax extracts. This shows that the ants are highly specific in the host tree and not even closely related trees can a pass. However, it also verified that the chemical that tells the ants which tree is which is located in the wax substance coating the leafs.
Source: "Rainforest Species Use Chemicals to Identify Which Plants to Prune." Red Orbit. RedOrbit. 13 May. 2011. Web. 13 May. 2011.
Image Source: Nijenhuis, Peter. "Heavy Load." Flickr. June, 2006. Web. 13 May. 2011.